Determination of reducing carbohydrates in natural honey samples by optical micrometry method

The present article deals with the determination of total amount glucose and fructose in natural honey samples by optical micrometry (OM) method. (Poly)vinyl alcohol spherical granules impregnated a 0.05 mol/l borax solution were chosen as sensitive element. It was shown that formation chelate esters boron polymer carbohydrates is pH-dependent process, pH range 8.5–10.0 most appropriate for quantifying reducing because are undiscriminated. not to sucrose presence due absence cis-diol fragments it. Subsequently, OM method tested analysis samples. relative standart deviation case less than 6%, results similar those obtained iodometric titration This makes suitable laboratory-scale applications.